The induced ROS production of leukocytes was measured in whole blood. The superoxide anion
production was induced with 0.2 μg/ml phorbol 12-miristate 13-acetate (PMA) (Sigma-Aldrich kft, Budapest,
Hungary), and was detected with luminol 3.33 μg/ml (Boehringer Gmbh Mannheim, Germany)
on a Chrono-log 560-VS lumino-aggregometer (Chrono-log Corp., USA). We have registered the maximum
rate of ROS production.
The phorbol–miristate–acetate (PMA) induced maximal ROS production of the leukocytes shows the
activation level of them. The results are demonstrated on Fig. 5. We have found a significant elevation
in ROS production in the non-postconditioned group (IR) before and after the ischaemia–reperfusion
period (2.59 ± 0.95 AU/103 cells vs. 5.7 ± 0.96 AU/103 cells, p < 0.05). In the postconditioned group
there was no significant change detected (3.89 ± 0.94 AU/103 cells vs. 4.63 ± 0.69 AU/103 cells).
请各位大侠指教ROS production 怎么检测？AU/103 cells是什么单位描述？谢谢！
