ysis of Neuropeptides by Liquid Chromatography and Mass Spectrometry
ysis of Neuropeptides by Liquid Chromatography and Mass Spectrometry (Techniques and instrumentation in ytical chemistry)
Publisher: Elsevier Science Ltd (September 1984) | IN-10: 0444424180 | 254 pages | PDF | 9.36 MB
PREFACE
"Such chemistries reside in the night darkness of the molecule that seems
to think ,
The Striders, Loren Eiseley
Loren Eiseley, quoted from "The Striders" in Notes of an Alchemist.
Copyright 1972 Loren Eiseley. Used with permission of Charles
Scribner's Sons.
The brain is one of the most exciting and fertile frontiers of scientific
study. A significant amount of time, experimentation, money, and effort is
being expended in a multi-faceted scientific research effort to unravel the
complexities of the brain. Some of the areas which are being studied include:
how the brain receives, processes, and sends information; how pain is dealt
with in terms of i t s recognition and i t s reduction; anatomical interconnections
among and within brain regions; memory - i t s initial recording, storage, and
recall: creative thought; movement disorders; neuroendocrinology :
neuropsychiatry; dementia; among many other aspects.
Such an area of difficult experimental research necessarily brings into
play a wide variety of basic and clinical scientific disciplines,
subdisciplines, and experimental techniques which involves biologists, medical
personnel, dentists, neurologists, neurochemists, psychiatrists, psychologists,
and ytical chemists. Answers sought to questions regarding the brain's
functioning require elucidation of the molecular processes that operate within
the various brain regions. Only by knowing several aspects of this problem,
such as which molecules are involved, their changing concentrations during
specific physiological events, their precursors and metabolites, and the
enzymatic and regulatory steps which are involved in those transformations,
can we treat the human organi in the normal and pathological states in any
rational and objective manner. Underpinning all of these research efforts are
appropriate ytical tools and techniques which are used to measure the
molecules involved with the maximum level of accuracy, precision, sensitivity,
and molecular specificity. Accuracy is demanded in these four critical
experimental parameters to avoid any confusion or ambiguity in our
experiments, conclusions, hypotheses, experiments, protocols, and
treatments.
Three of the most explosively growing fields in science today include
biologically important peptides, high performance liquid chromatography
(HPLC), and mass spectrometry (MS). Because of the significant advances in
the field of neurobiology , and particular neuropeptides, there now exists
an increasingly critical need for dependable structural information. The
timing is appropriate to review here the pertinent features of these three
areas and to bring into a novel juxtaposition the mutually beneficial aspects
of these areas.
This book is basically oriented towards a review of the literature, but
in those cases where i t is required, more in-depth references will be
suggested to the reader. Papers are selected from the wide spectrum of
available research, briefly reviewed, and presented here to indicate the
panorama of research that is being conducted and to point the way to needed
research,
An effort has been made to remain up-to-date with respect to the
current scientific literature, Because the three fields being discussed
represent some of the most rapidly expanding fields in science today, this
goal is rather difficult to achieve. Towards resolution of this dilemma, a
camera-ready copy is prepared as opposed to a typeset book
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